Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus)
Identifieur interne : 002352 ( Main/Exploration ); précédent : 002351; suivant : 002353Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus)
Auteurs : Marko Zivcec [États-Unis, Canada] ; David Safronetz [États-Unis] ; Elaine Haddock [États-Unis] ; Heinz Feldmann [États-Unis, Canada] ; Hideki Ebihara [États-Unis]Source :
- Journal of immunological methods [ 0022-1759 ] ; 2011.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
The Syrian golden hamster (Mesocricetus auratus) is a valuable but under-utilized animal model for studies of human viral pathogens such as bunyaviruses, arenaviruses, flaviviruses, henipaviruses, and SARS-coronavirus. A lack of suitable reagents and specific assays for monitoring host responses has limited the use of this animal model to clinical observations, pathology and humoral immune responses. The objective of this study was to establish and validate assays to monitor host immune responses in the hamster including important pro-inflammatory, anti-inflammatory and innate immune responses, as well as markers of apoptosis, cell proliferation, cell junction integrity and coagulation. Commercially available mouse and rat ELISA and luminex panels were screened for potential cross-reactivity, but were found to be of limited value for studying host responses in hamsters. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays for the detection of 51 immune-related and four internal reference genes were developed. To validate the immune-related assays, hamsters were infected with vesicular stomatitis virus (VSV), Indiana species, or treated with lipopolysaccharide (LPS) and host immune responses were monitored in selected organs. Ribosomal protein L18 was identified as the most stable internal reference gene. In conclusion, these new assays will greatly improve the use of the hamster as an important small animal model in infectious disease research.
Affiliations:
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Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, 903 S 4th Street</s1><s2>Hamilton, MT</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Montana</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Journal of immunological methods</title><title level="j" type="abbreviated">J. immunol. methods</title><idno type="ISSN">0022-1759</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of immunological methods</title><title level="j" type="abbreviated">J. immunol. methods</title><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene</term><term>Hamster</term><term>Immune response</term><term>Immunological method</term><term>Polymerase chain reaction</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Hamster</term><term>Réponse immune</term><term>Réaction chaîne polymérase</term><term>Gène</term><term>Méthode immunologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Syrian golden hamster (Mesocricetus auratus) is a valuable but under-utilized animal model for studies of human viral pathogens such as bunyaviruses, arenaviruses, flaviviruses, henipaviruses, and SARS-coronavirus. A lack of suitable reagents and specific assays for monitoring host responses has limited the use of this animal model to clinical observations, pathology and humoral immune responses. The objective of this study was to establish and validate assays to monitor host immune responses in the hamster including important pro-inflammatory, anti-inflammatory and innate immune responses, as well as markers of apoptosis, cell proliferation, cell junction integrity and coagulation. Commercially available mouse and rat ELISA and luminex panels were screened for potential cross-reactivity, but were found to be of limited value for studying host responses in hamsters. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays for the detection of 51 immune-related and four internal reference genes were developed. To validate the immune-related assays, hamsters were infected with vesicular stomatitis virus (VSV), Indiana species, or treated with lipopolysaccharide (LPS) and host immune responses were monitored in selected organs. Ribosomal protein L18 was identified as the most stable internal reference gene. In conclusion, these new assays will greatly improve the use of the hamster as an important small animal model in infectious disease research.</div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Manitoba</li><li>Montana</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><country name="États-Unis"><region name="Montana"><name sortKey="Zivcec, Marko" sort="Zivcec, Marko" uniqKey="Zivcec M" first="Marko" last="Zivcec">Marko Zivcec</name></region><name sortKey="Ebihara, Hideki" sort="Ebihara, Hideki" uniqKey="Ebihara H" first="Hideki" last="Ebihara">Hideki Ebihara</name><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name><name sortKey="Safronetz, David" sort="Safronetz, David" uniqKey="Safronetz D" first="David" last="Safronetz">David Safronetz</name></country><country name="Canada"><region name="Manitoba"><name sortKey="Zivcec, Marko" sort="Zivcec, Marko" uniqKey="Zivcec M" first="Marko" last="Zivcec">Marko Zivcec</name></region><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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